Experiment	GSM3687217	Study	GSE128854 
Organism	Mus musculus (mm10)	Tissue	whole brain
Protocol	10X	Sample#	4
Sample Run	SRR8795645 SRR8795646 SRR8795647 SRR8795648
Reference	Van Hove, H., et al. (2019). A single-cell atlas of mouse brain macrophages reveals unique transcriptional identities shaped by ontogeny and tissue environment. Nature Neuroscience 22(6): 1021-1035.

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Gene#	15464	Cell#	4251
PolyA site#	scAPAtrap 14215Sierra 12001	Gene with polyA site#	scAPAtrap 11609Sierra 9445
APA event#	scAPAtrap 4670Sierra 4467	Gene with APA event#	scAPAtrap 2064Sierra 1922
Cell types 5	CD8+ T cell: 777Microglial cell: 1046Transit amplifying (TA) cell: 1980Hematopoetic cell: 269CXCR6+ T cell: 179

APA index

PolyA Site Expression APA Usage Weighted 3' UTR Length

Tools

scAPAtrap Sierra

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Box plot showing in different cell types

UMAP showing 2D embeddings of cell types based on the gene expression profile

UMAP showing distribution in single cells

Scatter plot showing correlation between gene expression and