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data6
Basic Information
Experiment
data6
Study
data6
Organism
Homo sapiens
(GRCh38)
Tissue
PBMC
Protocol
10X
Sample#
1
Sample Run
-
Reference
10k Human PBMCs Multiplexed, 2 CMOs - Human 2
Gene#
18552
Cell#
6009
PolyA site#
scAPAtrap
12829
Sierra
5779
Gene with polyA site#
scAPAtrap
9942
Sierra
4907
APA event#
scAPAtrap
4909
Sierra
1545
Gene with APA event#
scAPAtrap
2022
Sierra
673
Cell types
10
B cell: 542
CD14+CD16+ Monocytes: 1968
Naive T cell: 1464
NK cell: 224
T cell: 796
Macrophage: 258
CD8+ T cell: 391
Megakaryocyte progenitor cell: 210
Plasmacytoid dendritic cell: 61
Monocyte derived dendritic cell: 95
APA information
APA index
PolyA Site Expression
APA Usage
Weighted 3' UTR Length
PolyA Site Expression
APA Usage
Weighted 3' UTR Length
Tools
scAPAtrap
Sierra
scAPAtrap
Sierra
Download
Box plot showing
poly(A) site expression
in different cell types
2
4
6
8
B cell
CD14+CD16+ Monocytes
Naive T cell
NK cell
T cell
Macrophage
CD8+ T cell
Megakaryocyte progenitor cell
Plasmacytoid dendritic cell
Monocyte derived dendritic cell
B cell
CD14+CD16+ Monocytes
Naive T cell
NK cell
T cell
Macrophage
CD8+ T cell
Megakaryocyte progenitor cell
Plasmacytoid dendritic cell
Monocyte derived dendritic cell
Poly(A) site expression
plotly-logomark
UMAP showing 2D embeddings of cell types based on the gene expression profile
−10
−5
0
5
10
−15
−10
−5
0
5
10
B cell
CD14+CD16+ Monocytes
Naive T cell
NK cell
T cell
Macrophage
CD8+ T cell
Megakaryocyte progenitor cell
Plasmacytoid dendritic cell
Monocyte derived dendritic cell
UMAP_1
UMAP_2
plotly-logomark
UMAP showing distribution
poly(A) site expression
in single cells
−10
−5
0
5
10
−15
−10
−5
0
5
10
2
4
6
8
Poly(A) site expression
UMAP_1
UMAP_2
plotly-logomark
Scatter plot showing correlation between gene expression and
poly(A) site expression
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
9
Gene expression
Poly(A) site expression
plotly-logomark