Experiment	data6	Study	data6 
Organism	Homo sapiens (GRCh38)	Tissue	PBMC
Protocol	10X	Sample#	1
Sample Run	-
Reference	10k Human PBMCs Multiplexed, 2 CMOs - Human 2

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Gene#	18552	Cell#	6009
PolyA site#	scAPAtrap 12829Sierra 5779	Gene with polyA site#	scAPAtrap 9942Sierra 4907
APA event#	scAPAtrap 4909Sierra 1545	Gene with APA event#	scAPAtrap 2022Sierra 673
Cell types 10	B cell: 542CD14+CD16+ Monocytes: 1968Naive T cell: 1464NK cell: 224T cell: 796Macrophage: 258CD8+ T cell: 391Megakaryocyte progenitor cell: 210Plasmacytoid dendritic cell: 61Monocyte derived dendritic cell: 95

APA index

PolyA Site Expression APA Usage Weighted 3' UTR Length

Tools

scAPAtrap Sierra

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Box plot showing in different cell types

UMAP showing 2D embeddings of cell types based on the gene expression profile

UMAP showing distribution in single cells

Scatter plot showing correlation between gene expression and