Experiment	data5	Study	data5 
Organism	Homo sapiens (GRCh38)	Tissue	PBMC
Protocol	10X	Sample#	1
Sample Run	-
Reference	10k Human PBMCs Multiplexed, 2 CMOs - Human 1

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Gene#	18746	Cell#	6424
PolyA site#	scAPAtrap 12960Sierra 6033	Gene with polyA site#	scAPAtrap 9938Sierra 5099
APA event#	scAPAtrap 5156Sierra 1643	Gene with APA event#	scAPAtrap 2134Sierra 709
Cell types 12	Naive T cell: 1594CD14+ Monocytes: 1301T cell: 888CD14+CD16+ Monocytes: 809Macrophage: 248CD8+ T cell: 395Plasmacytoid dendritic cell: 56B cell: 560NK cell: 238T helper17 (Th17) cell: 61Megakaryocyte progenitor cell: 159Monocyte derived dendritic cell: 115

APA index

PolyA Site Expression APA Usage Weighted 3' UTR Length

Tools

scAPAtrap Sierra

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Box plot showing in different cell types

UMAP showing 2D embeddings of cell types based on the gene expression profile

UMAP showing distribution in single cells

Scatter plot showing correlation between gene expression and