Experiment	data25	Study	data25 
Organism	Homo sapiens (GRCh38)	Tissue	PBMC
Protocol	10X	Sample#	1
Sample Run	-
Reference	4k PBMCs from a Healthy Donor

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Gene#	16655	Cell#	4340
PolyA site#	scAPAtrap 15108Sierra 13807	Gene with polyA site#	scAPAtrap 11940Sierra 10231
APA event#	scAPAtrap 5568Sierra 6042	Gene with APA event#	scAPAtrap 2400Sierra 2477
Cell types 10	CD14+ Monocytes: 910T cell: 575Naive T cell: 1202Plasmacytoid dendritic cell: 37Monocyte derived dendritic cell: 125B cells naive: 409CD8+ T cell: 573B cell: 203Granulocyte: 159Macrophage: 147

APA index

PolyA Site Expression APA Usage Weighted 3' UTR Length

Tools

scAPAtrap Sierra

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Box plot showing in different cell types

UMAP showing 2D embeddings of cell types based on the gene expression profile

UMAP showing distribution in single cells

Scatter plot showing correlation between gene expression and