Experiment	data18	Study	data18 
Organism	Homo sapiens (GRCh38)	Tissue	PBMC
Protocol	10X	Sample#	1
Sample Run	-
Reference	5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor with cell surface proteins (v3 chemistry)

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Gene#	17802	Cell#	5197
PolyA site#	scAPAtrap 11779Sierra 9252	Gene with polyA site#	scAPAtrap 9756Sierra 7516
APA event#	scAPAtrap 3612Sierra 3040	Gene with APA event#	scAPAtrap 1589Sierra 1309
Cell types 10	Naive T cell: 1948T cell: 664CD14+CD16+ Monocytes: 603CD14+ Monocytes: 687Macrophage: 118Monocyte derived dendritic cell: 162B cell: 342NK cell: 298CD8+ T cell: 344Plasmacytoid dendritic cell: 31

APA index

PolyA Site Expression APA Usage Weighted 3' UTR Length

Tools

scAPAtrap Sierra

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Box plot showing in different cell types

UMAP showing 2D embeddings of cell types based on the gene expression profile

UMAP showing distribution in single cells

Scatter plot showing correlation between gene expression and