Experiment	data16	Study	data16 
Organism	Homo sapiens (GRCh38)	Tissue	PBMC
Protocol	10X	Sample#	1
Sample Run	-
Reference	5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v3 chemistry)

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Gene#	18791	Cell#	4962
PolyA site#	scAPAtrap 16395Sierra 14817	Gene with polyA site#	scAPAtrap 12351Sierra 10660
APA event#	scAPAtrap 6852Sierra 6874	Gene with APA event#	scAPAtrap 2808Sierra 2728
Cell types 10	Monocyte derived dendritic cell: 380T cell: 1469B cell: 565Naive T cell: 1378CD8+ T cell: 352CD14+ Monocytes: 652Megakaryocyte progenitor cell: 30Macrophage: 86CD14+CD16+ Monocytes: 24Plasmacytoid dendritic cell: 26

APA index

PolyA Site Expression APA Usage Weighted 3' UTR Length

Tools

scAPAtrap Sierra

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Box plot showing in different cell types

UMAP showing 2D embeddings of cell types based on the gene expression profile

UMAP showing distribution in single cells

Scatter plot showing correlation between gene expression and