Experiment	data15	Study	data15 
Organism	Homo sapiens (GRCh38)	Tissue	PBMC
Protocol	10X	Sample#	1
Sample Run	-
Reference	Peripheral blood mononuclear cells (PBMCs) from a healthy donor - Manual (channel 5)

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Gene#	17516	Cell#	4035
PolyA site#	scAPAtrap 10715Sierra 10369	Gene with polyA site#	scAPAtrap 9144Sierra 8096
APA event#	scAPAtrap 2849Sierra 3875	Gene with APA event#	scAPAtrap 1278Sierra 1612
Cell types 8	Naive T cell: 2346CD8+ T cell: 144CD14+ Monocytes: 731Plasmacytoid dendritic cell: 74B cell: 509NK cell: 178CD14+CD16+ Monocytes: 25Macrophage: 28

APA index

PolyA Site Expression APA Usage Weighted 3' UTR Length

PolyA Site Expression

APA Usage

Weighted 3' UTR Length

Tools

scAPAtrap Sierra

scAPAtrap

Sierra

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Box plot showing poly(A) site expression in different cell types

UMAP showing 2D embeddings of cell types based on the gene expression profile

UMAP showing distribution poly(A) site expression in single cells

Scatter plot showing correlation between gene expression and poly(A) site expression